Electricity generation and real oily wastewater treatment by Pseudomonas citronellolis 620C in a microbial fuel cell: pyocyanin production as electron shuttle.

In the present study, the potential of Pseudomonas citronellolis 620C strain was evaluated, for the first time, to generate electricity in a standard, double chamber microbial fuel cell (MFC), with oily wastewater (OW) being the fuel at 43.625 mg/L initial chemical oxygen demand (COD). Both electrochemical and physicochemical results suggested that this P. citronellolis strain utilized efficiently the OW substrate and generated electricity in the MFC setup reaching 0.05 mW/m2 maximum power. COD removal was remarkable reaching 83.6 ± 0.1%, while qualitative and quantitative gas chromatography/mass spectrometry (GC/MS) analysis of the OW total petroleum and polycyclic aromatic hydrocarbons, and fatty acids revealed high degradation capacity. It was also determined that P. citronellolis 620C produced pyocyanin as electron shuttle in the anodic MFC chamber. To the authors' best knowledge, this is the first study showing (phenazine-based) pyocyanin production from a species other than P. aeruginosa and, also, the first time that P. citronellolis 620C has been shown to produce electricity in a MFC. The production of pyocyanin, in combination with the formation of biofilm in the MFC anode, as observed with scanning electron microscopy (SEM) analysis, makes this P. citronellolis strain an attractive and promising candidate for wider MFC applications.

Nanofiber applications in microbial fuel cells for enhanced energy generation: a mini review.

Microbial fuel cells (MFCs) represent simple devices that harness the metabolic activities of microorganisms to produce electrical energy from diverse sources such as organic waste and sustainable biomass. Because of their unique advantage to generate sustainable energy, through the employment of biodegradable and repurposed waste materials, the development of MFCs has garnered considerable interest. Critical elements are typically the electrodes and separator. This mini-review article presents a critical assessment of nanofiber technology used as electrodes and separators in MFCs to enhance energy generation. In particular, the review highlights the application of nanofiber webs in each part of MFCs including anodes, cathodes, and membranes and their influence on energy generation. The role of nanofiber technology in this regard is then analysed in detail, focusing on improved electron transfer rate, enhanced biofilm formation, and enhanced durability and stability. In addition, the challenges and opportunities associated with integrating nanofibers into MFCs are discussed, along with suggestions for future research in this field. Significant developments in MFCs over the past decade have led to a several-fold increase in achievable power density, yet further improvements in performance and the exploration of cost-effective materials remain promising areas for further advancement. This review demonstrates the great promise of nanofiber-based electrodes and separators in future applications of MFCs.

Energy harvesting from plants using hybrid microbial fuel cells; potential applications and future exploitation.

Microbial Fuel Cells (MFC) can be fuelled using biomass derived from dead plant material and can operate on plant produced chemicals such as sugars, carbohydrates, polysaccharides and cellulose, as well as being "fed" on a regular diet of primary biomass from plants or algae. An even closer relationship can exist if algae (e.g., prokaryotic microalgae or eukaryotic and unicellular algae) can colonise the open to air cathode chambers of MFCs driving photosynthesis, producing a high redox gradient due to the oxygenic phase of collective algal cells. The hybrid system is symbiotic; the conditions within the cathodic chamber favour the growth of microalgae whilst the increased redox and production of oxygen by the algae, favour a more powerful cathode giving a higher maximum voltage and power to the photo-microbial fuel cell, which can ultimately be harvested for a range of end-user applications. MFCs can utilise a wide range of plant derived materials including detritus, plant composts, rhizodeposits, root exudates, dead or dying macro- or microalgae, via Soil-based Microbial Fuel Cells, Sediment Microbial Fuel Cells, Plant-based microbial fuel cells, floating artificial islands and constructed artificial wetlands. This review provides a perspective on this aspect of the technology as yet another attribute of the benevolent Bioelectrochemical Systems.

Effects of municipal waste compost on microbial biodiversity and energy production in terrestrial microbial fuel cells.

Microbial Fuel Cells (MFCs) transform organic matter into electricity through microbial electrochemical reactions catalysed on anodic and cathodic half-cells. Terrestrial MFCs (TMFCs) are a bioelectrochemical system for bioelectricity production as well as soil remediation. In TMFCs, the soil is the ion-exchange electrolyte, whereas a biofilm on the anode oxidises organic matter through electroactive bacteria. Little is known of the overall microbial community composition in a TMFC, which impedes complete exploitation of the potential to generate energy in different soil types. In this context, an experiment was performed to reveal the prokaryotic community structure in single chamber TMFCs with soil in the presence and absence of a municipal waste compost (3% w/v). The microbial community was assessed on the anode and cathode and in bulk soil at the end of the experiment (54 days). Moreover, TMFC electrical performance (voltage and power) was also evaluated over the experimental period, varying the external resistance to improve performance. Compost stimulated soil microbial activity, in line with a general increase in voltage and power. Significant differences were observed in the microbial communities between initial soil conditions and TMFCs, and between the anode, cathode and bulk soil in the presence of the compost. Several electroactive genera (Bacillus, Fulvivirga, Burkholdeira and Geobacter) were found at the anode in the presence of compost. Overall, the use of municipal waste compost significantly increased the performance of the MFCs in terms of electrical power and voltage generated, not least thanks to the selective pressure towards electroactive bacteria on the anode.

Prevention and removal of membrane and separator biofouling in bioelectrochemical systems: a comprehensive review.

Bioelectrochemical systems (BESs) have made significant progress in recent years in all aspects of their technology. BESs usually work with a membrane or a separator, which is one of their most critical components affecting performance. Quite often, biofilm from either the anolyte or catholyte forms on the membrane, which can negatively affect its performance. In critical cases, the long-term power performance observed for microbial fuel cells (MFCs) has dropped by over 90%. Surface modification and composite material approaches as well as chemical and physical cleaning techniques involving surfactants, acids, hydroxides, and ultrasounds have been successfully implemented to combat biofilm formation. Surface modifications produced up to 6-7 times higher power performance in the long-term, whereas regeneration strategies resulted in up to 100% recovery of original performance. Further studies include tools such as fluid dynamics-based design and plasma cleaning. The biofouling area is still underexplored in the field of bioelectrochemistry and requires systematic improvement. Therefore, this review summarizes the most recent knowledge with the aim of helping the research and engineering community select the best strategy and discuss further perspectives for combating the undesirable biofilm.

Microbial fuel cell compared to a chemostat.

Microbial Fuel Cells (MFCs) represent a green and sustainable energy conversion system that integrate bacterial biofilms within an electrochemical two-electrode set-up to produce electricity from organic waste. In this review, we focus on a novel exploratory model, regarding "thin" biofilms forming on highly perfusable (non-diffusible) anodes in small-scale, continuous flow MFCs due to the unique properties of the electroactive biofilm. We discuss how this type of MFC can behave as a chemostat in fulfilling common properties including steady state growth and multiple steady states within the limit of biological physicochemical conditions imposed by the external environment. With continuous steady state growth, there is also continuous metabolic rate and continuous electrical power production, which like the chemostat can be controlled. The model suggests that in addition to controlling growth rate and power output by changing the external resistive load, it will be possible instead to change the flow rate/dilution rate.

Microbial fuel cell scale-up options: Performance evaluation of membrane (c-MFC) and membrane-less (s-MFC) systems under different feeding regimes.

In recent years, bioelectrochemical systems have advanced towards upscaling applications and tested during field trials, primarily for wastewater treatment. Amongst reported trials, two designs of urine-fed microbial fuel cells (MFCs) were tested successfully on a pilot scale as autonomous sanitation systems for decentralised area. These designs, known as ceramic MFCs ( c -MFCs) and self-stratifying MFCs ( s -MFC), have never been calibrated under similar conditions. Here, the most advanced versions of both designs were assembled and tested under similar feeding conditions. The performance and efficiency were evaluated under different hydraulic retention times (HRT), through chemical oxygen demand measures and polarisation experiments. Results show that c -MFCs displayed constant performance independently from the HRT (32.2 ± 3.9 W m-3) whilst displaying high energy conversion efficiency at longer HRT (NER COD  = 2.092 ± 0.119 KWh.Kg COD -1, at 24h HRT). The s -MFC showed a correlation between performance and HRT. The highest performance was reached under short HRT (69.7 ± 0.4 W m-3 at 3h HRT), but the energy conversion efficiency was constant independently from the HRT (0.338 ± 0.029 KWh.Kg COD -1). The c -MFCs and s -MFCs similarly showed the highest volumetric efficiency under long HRT (65h) with NER V of 0.747 ± 0.010 KWh.m-3 and 0.825 ± 0.086 KWh.m-3, respectively. Overall, c -MFCs seems more appropriate for longer HRT and s -MFCs for shorter HRT.

Microbial Fuel Cell Based Thermosensor for Robotic Applications.

On the roadmap to building completely autonomous artificial bio-robots, all major aspects of robotic functions, namely, energy generation, processing, sensing, and actuation, need to be self-sustainable and function in the biological realm. Microbial Fuel Cells (MFCs) provide a platform technology for achieving this goal. In a series of experiments, we demonstrate that MFCs can be used as living, autonomous sensors in robotics. In this work, we focus on thermal sensing that is akin to thermoreceptors in mammalian entities. We therefore designed and tested an MFC-based thermosensor system for utilization within artificial bio-robots such as EcoBots. In open-loop sensor characterization, with a controlled load resistance and feed rate, the MFC thermoreceptor was able to detect stimuli of 1 min directed from a distance of 10 cm causing a temperature rise of ∼1°C at the thermoreceptor. The thermoreceptor responded to continuous stimuli with a minimum interval of 384 s. In a practical demonstration, a mobile robot was fitted with two artificial thermosensors, as environmental thermal detectors for thermotactic application, mimicking thermotaxis in biology. In closed-loop applications, continuous thermal stimuli were detected at a minimum time interval of 160 s, without the need for complete thermoreceptor recovery. This enabled the robot to detect thermal stimuli and steer away from a warmer thermal source within the rise of 1°C. We envision that the thermosensor can be used for future applications in robotics, including as a potential sensor mechanism for maintaining thermal homeostasis.

Microbial fuel cells and their electrified biofilms.

Bioelectrochemical systems (BES) represent a wide range of different biofilm-based bioreactors that includes microbial fuel cells (MFCs), microbial electrolysis cells (MECs) and microbial desalination cells (MDCs). The first described bioelectrical bioreactor is the Microbial Fuel Cell and with the exception of MDCs, it is the only type of BES that actually produces harvestable amounts of electricity, rather than requiring an electrical input to function. For these reasons, this review article, with previously unpublished supporting data, focusses primarily on MFCs. Of relevance is the architecture of these bioreactors, the type of membrane they employ (if any) for separating the chambers along with the size, as well as the geometry and material composition of the electrodes which support biofilms. Finally, the structure, properties and growth rate of the microbial biofilms colonising anodic electrodes, are of critical importance for rendering these devices, functional living 'engines' for a wide range of applications.

Effect of simple interventions on the performance of a miniature MFC fed with fresh urine.

The aim of the present study is to enhance the performance of a microbial fuel cell (MFC) design by making simple interventions. Specifically, terracotta "t" and mullite "m" ceramics are tested as membranes while carbon veil and carbon cloth are used as electrodes. In the case of "m" cylinders different dimensions are examined (m: ID 30 mm x height 11.5 mm; sm: ID 18 mm x height 18 mm). The units operated continuously with urine as the feedstock. The best performing is the sm type (60-100 µW), followed by the t type (40-80 µW) and the m type (20-40 µW). Polarisation experiments indicated that activated carbon on the anode enhances the power output (t: 423 µW, sm: 288 µW). Similarly, the increase of the surface area and the addition of stainless steel mesh on the cathode improves the power performance for the "sm" and the "t" units. Furthermore, it is shown that the design with the smaller internal diameter, performs better and is more stable through time.

Electronic faucet powered by low cost ceramic microbial fuel cells treating urine.

Hygienic measures are extremely important to avoid the transmission of contagious viruses and diseases. The use of an electronic faucet increases the hygiene, encourages hand washing, avoids touching the faucet for opening and closing, and it saves water, since the faucet is automatically closed. The microbial fuel cell (MFC) technology has the capability to convert environmental waste into energy. The implementation of low cost ceramic MFCs into electronic interfaces integrated in toilets, would offer a compact powering system as well as an environmentally friendly small-scale treatment plant. In this work, the use of low cost ceramic MFCs to power an L20-E electronic faucet is presented for the first time. A single MFC was capable of powering an electronic faucet with an open/close cycle of 8.5 min, with 200 ml of urine. With a footprint of 360 cm3, the MFC could easily be integrated in a toilet. The possibility to power e-toilet components with MFCs offers a sustainable energy generation system. Other electronic components including an automatic flush, could potentially be powered by MFCs and contribute to the maintenance efficiency and hygiene of the public toilets, leading to a new generation of self-sustained energy recovering e-toilets.

Electrosynthesis, modulation, and self-driven electroseparation in microbial fuel cells.

Microbial electrosynthesis (MES) represents a sustainable platform that converts waste into resources, using microorganisms within an electrochemical cell. Traditionally, MES refers to the oxidation/reduction of a reactant at the electrode surface with externally applied potential bias. However, microbial fuel cells (MFCs) generate electrons that can drive electrochemical reactions at otherwise unbiased electrodes. Electrosynthesis in MFCs is driven by microbial oxidation of organic matter releasing electrons that force the migration of cationic species to the cathode. Here, we explore how electrosynthesis can coexist within electricity-producing MFCs thanks to electro-separation of cations, electroosmotic drag, and oxygen reduction within appropriately designed systems. More importantly, we report on a novel method of in situ modulation for electrosynthesis, through additional "pin" electrodes. Several MFC electrosynthesis modulating methods that adjust the electrode potential of each half-cell through the pin electrodes are presented. The innovative concept of electrosynthesis within the electricity producing MFCs provides a multidisciplinary platform converting waste-to-resources in a self-sustainable way.

Neural Networks Predicting Microbial Fuel Cells Output for Soft Robotics Applications.

The development of biodegradable soft robotics requires an appropriate eco-friendly source of energy. The use of Microbial Fuel Cells (MFCs) is suggested as they can be designed completely from soft materials with little or no negative effects to the environment. Nonetheless, their responsiveness and functionality is not strictly defined as in other conventional technologies, i.e. lithium batteries. Consequently, the use of artificial intelligence methods in their control techniques is highly recommended. The use of neural networks, namely a nonlinear autoregressive network with exogenous inputs was employed to predict the electrical output of an MFC, given its previous outputs and feeding volumes. Thus, predicting MFC outputs as a time series, enables accurate determination of feeding intervals and quantities required for sustenance that can be incorporated in the behavioural repertoire of a soft robot.

Impact of Inoculum Type on the Microbial Community and Power Performance of Urine-Fed Microbial Fuel Cells.

Bacteria are the driving force of the microbial fuel cell (MFC) technology, which benefits from their natural ability to degrade organic matter and generate electricity. The development of an efficient anodic biofilm has a significant impact on the power performance of this technology so it is essential to understand the effects of the inoculum nature on the anodic bacterial diversity and establish its relationship with the power performance of the system. Thus, this work aims at analysing the impact of 3 different types of inoculum: (i) stored urine, (ii) sludge and (iii) effluent from a working MFC, on the microbial community of the anodic biofilm and therefore on the power performance of urine-fed ceramic MFCs. The results showed that MFCs inoculated with sludge outperformed the rest and reached a maximum power output of 40.38 mW·m-2anode (1.21 mW). The power performance of these systems increased over time whereas the power output by MFCs inoculated either with stored urine or effluent decreased after day 30. These results are directly related to the establishment and adaptation of the microbial community on the anode during the assay. Results showed the direct relationship between the bacterial community composition, originating from the different inocula, and power generation within the MFCs.

From the lab to the field: Self-stratifying microbial fuel cells stacks directly powering lights.

The microbial fuel cell (MFC) technology relies on energy storage and harvesting circuitry to deliver stable power outputs. This increases costs, and for wider deployment into society, these should be kept minimal. The present study reports how a MFC system was developed to continuously power public toilet lighting, with for the first time no energy storage nor harvesting circuitry. Two different stacks, one consisting of 15 and the other 18 membrane-less MFC modules, were operated for 6 days and fuelled by the urine of festival goers at the 2019 Glastonbury Music Festival. The 15-module stack was directly connected to 2 spotlights each comprising 6 LEDs. The 18-module stack was connected to 2 identical LED spotlights but going through 2 LED electronic controller/drivers. Twenty hours after inoculation the stacks were able to directly power the bespoke lighting system. The electrical energy produced by the 15-module stack evolved with usage from ≈280 mW (≈2.650 V at ≈105 mA) at the beginning to ≈860 mW (≈2.750 V at ≈300 mA) by the end of the festival. The electrical energy produced by the LED-driven 18-module stack increased from ≈490 mW at the beginning to ≈680 mW toward the end of the festival. During this period, illumination was above the legal standards for outdoor public areas, with the 15-module stack reaching a maximum of ≈89 Lx at 220 cm. These results demonstrate for the first time that the MFC technology can be deployed as a direct energy source in decentralised area (e.g. refugee camps).

Scaling up self-stratifying supercapacitive microbial fuel cell.

Self-stratifying microbial fuel cells with three different electrodes sizes and volumes were operated in supercapacitive mode. As the electrodes size increased, the equivalent series resistance decreased, and the overall power was enhanced (small: ESR = 7.2 Ω and P max  = 13 mW; large: ESR = 4.2 Ω and P max  = 22 mW). Power density referred to cathode geometric surface area and displacement volume of the electrolyte in the reactors. With regards to the electrode wet surface area, the large size electrodes (L-MFC) displayed the lowest power density (460 µW cm-2) whilst the small and medium size electrodes (S-MFC, M-MFC) showed higher densities (668 µW cm-2 and 633 µW cm-2, respectively). With regard to the volumetric power densities the S-MFC, the M-MFC and the L-MFC had similar values (264 µW mL-1, 265 µW mL-1 and 249 µW cm-1, respectively). Power density normalised in terms of carbon weight utilised for fabricating MFC cathodes-electrodes showed high output for smaller electrode size MFC (5811 µW g-1-C- and 3270 µW g-1-C- for the S-MFC and L-MFC, respectively) due to the fact that electrodes were optimised for MFC operations and not supercapacitive discharges. Apparent capacitance was high at lower current pulses suggesting high faradaic contribution. The electrostatic contribution detected at high current pulses was quite low. The results obtained give rise to important possibilities of performance improvements by optimising the device design and the electrode fabrication.

A new method for urine electrofiltration and long term power enhancement using surface modified anodes with activated carbon in ceramic microbial fuel cells.

This work is presenting for the first time the use of inexpensive and efficient anode material for boosting power production, as well as improving electrofiltration of human urine in tubular microbial fuel cells (MFCs). The MFCs were constructed using unglazed ceramic clay functioning as the membrane and chassis. The study is looking into effective anodic surface modification by applying activated carbon micro-nanostructure onto carbon fibres that allows electrode packing without excessive enlargement of the electrode. The surface treatment of the carbon veil matrix resulted in 3.7 mW (52.9 W m-3 and 1626 mW m-2) of power generated and almost a 10-fold increase in the anodic current due to the doping as well as long-term stability in one year of continuous operation. The higher power output resulted in the synthesis of clear catholyte, thereby i) avoiding cathode fouling and contributing to the active splitting of both pH and ions and ii) transforming urine into a purified catholyte - 30% salt reduction - by electroosmotic drag, whilst generating - rather than consuming - electricity, and in a way demonstrating electrofiltration. For the purpose of future technology implementation , the importance of simultaneous increase in power generation, long-term stability over 1 year and efficient urine cleaning by using low-cost materials, is very promising and helps the technology enter the wider market.

Air-breathing cathode self-powered supercapacitive microbial fuel cell with human urine as electrolyte.

In this work, a membraneless microbial fuel cell (MFC) with an empty volume of 1.5 mL, fed continuously with hydrolysed urine, was tested in supercapacitive mode (SC-MFC). In order to enhance the power output, a double strategy was used: i) a double cathode was added leading to a decrease in the equivalent series resistance (ESR); ii) the apparent capacitance was boosted up by adding capacitive features on the anode electrode. Galvanostatic (GLV) discharges were performed at different discharge currents. The results showed that both strategies were successful obtaining a maximum power output of 1.59 ± 0.01 mW (1.06 ± 0.01 mW mL-1) at pulse time of 0.01 s and 0.57 ± 0.01 mW (0.38 ± 0.01 mW mL-1) at pulse time of 2 s. The highest energy delivered at ipulse equal to 2 mA was 3.3 ± 0.1 mJ. The best performing SC-MFCs were then connected in series and parallel and tested through GLV discharges. As the power output was similar, the connection in parallel allowed to roughly doubling the current produced. Durability tests over ≈5.6 days showed certain stability despite a light overall decrease.

Developing 3D-Printable Cathode Electrode for Monolithically Printed Microbial Fuel Cells (MFCs).

Microbial Fuel Cells (MFCs) employ microbial electroactive species to convert chemical energy stored in organic matter, into electricity. The properties of MFCs have made the technology attractive for bioenergy production. However, a challenge to the mass production of MFCs is the time-consuming assembly process, which could perhaps be overcome using additive manufacturing (AM) processes. AM or 3D-printing has played an increasingly important role in advancing MFC technology, by substituting essential structural components with 3D-printed parts. This was precisely the line of work in the EVOBLISS project, which investigated materials that can be extruded from the EVOBOT platform for a monolithically printed MFC. The development of such inexpensive, eco-friendly, printable electrode material is described below. The electrode in examination (PTFE_FREE_AC), is a cathode made of alginate and activated carbon, and was tested against an off-the-shelf sintered carbon (AC_BLOCK) and a widely used activated carbon electrode (PTFE_AC). The results showed that the MFCs using PTFE_FREE_AC cathodes performed better compared to the PTFE_AC or AC_BLOCK, producing maximum power levels of 286 µW, 98 µW and 85 µW, respectively. In conclusion, this experiment demonstrated the development of an air-dried, extrudable (3D-printed) electrode material successfully incorporated in an MFC system and acting as a cathode electrode.

Complete Microbial Fuel Cell Fabrication Using Additive Layer Manufacturing.

Improving the efficiency of microbial fuel cell (MFC) technology by enhancing the system performance and reducing the production cost is essential for commercialisation. In this study, building an additive manufacturing (AM)-built MFC comprising all 3D printed components such as anode, cathode and chassis was attempted for the first time. 3D printed base structures were made of low-cost, biodegradable polylactic acid (PLA) filaments. For both anode and cathode, two surface modification methods using either graphite or nickel powder were tested. The best performing anode material, carbon-coated non-conductive PLA filament, was comparable to the control modified carbon veil with a peak power of 376.7 µW (7.5 W m-3) in week 3. However, PLA-based AM cathodes underperformed regardless of the coating method, which limited the overall performance. The membrane-less design produced more stable and higher power output levels (520-570 µW, 7.4-8.1 W m-3) compared to the ceramic membrane control MFCs. As the final design, four AM-made membrane-less MFCs connected in series successfully powered a digital weather station, which shows the current status of low-cost 3D printed MFC development.